三磷酸肌醇、钙离子在胃泌素促人结肠癌SW480细胞株增殖中的作用

Role of inositol 1,4,5 trisphosphate calcium in gastrin induced proliferation of human colon cell line SW480

目的：探讨胃泌素对人结肠癌细胞株ＳＷ４８０内三磷酸肌醇（ＩＰ３）、钙离子（Ｃａ２＋）的影响及其在细胞增殖中的作用，为结肠癌抗信息传导治疗提供实验依据。方法：①３Ｈ－肌醇标记细胞进行ＩＰ３分析。②应用Ｃａ２＋荧光指示剂Ｆｕｒａ－２检测细胞内Ｃａ２＋的浓度。③ＭＴＴ比色分析法检测活细胞数（吸光度Ａ值）。结果：５肽胃泌素（Ｐｅｎｔａｇａｓｔｒｉｎ，ＰＧ）可使ＳＷ４８０细胞内ＩＰ３、Ｃａ２＋水平升高，同时活细胞数Ａ值增加；５肽胃泌素与其受体拮抗剂丙谷胺（Ｐｒｏｇｌｕｍｉｄｅ，ＰＧＬ）合用时，癌细胞内ＩＰ３、Ｃａ２＋水平和活细胞数Ａ值均无明显变化。

Objective: To investigate the effects of gastrin on inositol 1,4,5 trisphosphate (IP3), intracellular calcium (Ca 2+ ) in human colon cancer cell line SW480 and its role in the proliferation of this cells line to provide experimental basis for development of antisignaling therapy for patients with colon cancer. Methods: 1) Level of IP3 was analyzed by myo inositol incorporation into inositol phospholipids in the cells. 2) Level of [Ca 2+ ] was measured by fluorescence measurement with fura 2/AM, a fluorescent index of calcium. 3) Viable cell count (VCC) of SW480 cells was calculated by MTT assay. Results: 1) Pentagastrin (PG) promoted the levels of IP3 and [Ca 2+ ] and increased VCC of SW480 cells. 2)When proglumide (PGL), a gastrin-receptor antagonist, used in combination with PG, no significant changes were found in the levels of IP3 and [Ca 2+ ] and increment of VCC of SW480 cells. Conclusion: Gastrin might promote the proliferation of SW480 cells through a receptor mediated inositol phospholipids signaling pathway.