急性肝衰竭大鼠肝组织组蛋白去乙酰化酶2的表达

Hep atic expression of histone deacetylase 2 in rats with acute liver failure

目的观察D-氨基半乳糖盐酸盐诱导急性肝衰竭模型大鼠在丙酮酸乙酯保护下,组蛋白去乙酰化酶2(HDAC2)的表达情况。方法将78只雄性Wistar大鼠随机分成正常组、模型组、EP干预组和EP治疗组。采用免疫组织化学法检测肝组织HDAC2表达;采用ELISA法检测肝组织匀浆HDAC2水平。结果与正常组比,模型组HDAC2的表达量减少(P<0.05),其中造模12h和24h肝组织HDAC2表达的IOD/Area值分别为0.0817±0.0118和0.0726±0.0111,明显低于正常组(0.1125±0.0047,P<0.01);EP干预组和治疗组与模型组间HDAC2的表达无明显差异(P>0.05)。结论 EP虽然对肝脏有保护作用,但不能影响HDAC2的表达。HDAC2的减少在急性肝衰竭的进展过程中,可能发挥重要的作用。

Objective To observe the hepatic expression of histone deacetylase 2（HDAC2）with the treatment of ethyl pyruvate（EP）in rats with acute live failure induced by D-galactosamine hydrochlorate.Methods 78 male Wistar rats were divided randomly as normal,model,intervention of EP and EP treatment groups.The expressions and levels of HDAC2 in liver tissues were detected by immunohistochemistry and ELISA,respectively.Results Compared with normal group,the expression of HDAC2 in model group decreased（P0.05）;Of which the IOD/Area values at 12h and 24h were 0.0817±0.0118 and 0.0726±0.0111,much lower than that in normal group （0.1125±0.0047,P0.01）.Conclusion The expressions of HDAC2 in rats with acute live failure were remarkably reduced.Although EP could protect the liver,it has no effect on the expression of HDAC2.