多烯磷脂酰胆碱在酒精性肝损伤铁过度沉积中的作用

The effect of polyene phosphatidylcholine on hepatic iron overload in rats with alcoholic liver damage

目的观察多烯磷脂酰胆碱对酒精性肝病大鼠铁稳态的影响,并对其作用机制作初步的探讨。方法将30只大鼠随机分为对照组、模型组和治疗组,分别喂饲Lieber-Decarli无酒精和含酒精液体6周。治疗组动物给予多烯磷脂酰胆碱干预。实验结束时检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、丙二醛(MDA)、铁、总铁结合力(TIBC)、铁蛋白(ferritin)和Hepcidin;大鼠肝组织行HE染色、普鲁士蓝铁染色及免疫组化染色检测Hepcidin。结果各组间血清铁、总铁结合力无显著性差异(P>0.05);模型组大鼠血清ALT、AST和Ferritin分别为37.9±14.3U/L、55.0±18.6U/L和337.8±132.8ng/ml,较对照组升高(P<0.05),而血清Hepcidin为124.1±32.0ng/ml,明显低于对照组(P=0.034);治疗组动物血清MDA为3.6±2.4nmol/ml,较模型组明显降低(P=0.01);组织学检查显示模型组肝细胞脂肪变明显,肝脏Hepcidin表达低于对照组(P<0.05);治疗组脂肪变细胞所占比例低于模型组,但肝脏Hepcidin蛋白表达量较模型组无统计学差异(P>0.05);对照组、模型组和治疗组肝脏铁颗粒数无统计学差异(P>0.05)。结论酒精摄入能够下调肝脏Hepcidin表达,导致酒精性肝损伤及铁稳态失衡。多烯磷脂酰胆碱通过抑制脂质过氧化而具有保护肝脏细胞的作用。

Objective To study the effects of polyene phosphatidylcholine on iron homeostaisis in rats with alcoholic liver disease.Methods Thirty wistar rats were randomly divided into control,model and intervention group.The animals were feeded with or without Lieber Decarli liquid in twenty and polyene phosphatidylcholine was given in ten rats.The serum alanine aminotransferase （ALT）,aspartate aminotransferase（AST）,malonyldialdehyde （MDA）,iron,total iron binding capacity （TIBC）,ferritin and hepcidin were examined after 6 weeks.Hepatic tissue was assessed by hematoxylin and eosin staining,prussian blue iron staining and immunohistochemisty staining.Results There were no significant differences in serum concentration of iron and TIBC among the three groups （P0.05）;the levels of serum ALT,AST and ferritin in the model group were respectively 37.9±14.3 U/L,55.0±18.6 U/L and 337.8±132.8 ng/ml,much higher than that in control group （P0.05,respectively）;the level of serum hepcidin was 124.1±32.0 ng/ml,much lower than in contro（lP=0.034）;the level of serum MDA in the intervention group was 3.6±2.4 nmol/ml,much lower than that in the model group （P=0.01）;there was no difference in liver iron content among the three groups.Conclusion Alcohol can lead to liver injury and iron overload,and decrease hepcidin expression in liver.Polyene phosphatidylcholine can protect alcohol liver damage by lipid hydroperoxides.