HPLC法测感冒清热颗粒中葛根素含量

Determanition of puerarin in Ganmao Qingre Granules by HPLC

目的测定感冒清热颗粒中葛根素的含量。方法采用高效液相法,色谱柱：Hypersil C18（4.6mm×250mm,5μm）,甲醇-水（30∶70）为流动相,流速1mL·min-1;检测波长为250nm。结果葛根素在进样量为0.0757~0.757μg范围内与峰面积呈良好的线性关系,平均回收率为100.8%,RSD值为1.06%。结论该法可用于感冒清热颗粒中葛根素的含量测定。

OBJECTIVE To determine puerarin in Ganmao Qingre Granules;METHODS The HPLC analysis was carried out on Hypersil C18（5μm,4.6mm ×250mm）,the mixture of methanol-water（30∶70）was used as the mobile phase,the flow rate was 1mL·min-1,the detection wavelength was 250nm.RESULTS The linear range of puerarin was 0.0757～0.757μg,the average recovery was 100.8% and the RSD was 1.06%.CONCLUSION The method can be applied in determanition of puerarin in Ganmao Qingre Granules.