p27在人睾丸生殖细胞肿瘤中的表达和意义

Expression and Biological Significance of p27 in Human Testicular Germ Cell Tumors

目的:检测p27蛋白在睾丸生殖细胞肿瘤、正常睾丸和慢性睾丸炎中的表达,探讨其临床意义。方法:采用免疫组化S-P法对55例睾丸生殖细胞肿瘤,10例正常睾丸组织和10例慢性睾丸炎组织中的p27蛋白的表达进行检测。结果:p27蛋白在55例睾丸生殖细胞肿瘤中阳性表达26例,阳性表达率为47.3%;在正常睾丸组织中阳性表达9例,阳性表达率为90.0%;慢性睾丸炎组织中阳性表达8例,阳性表达率为80.0%。睾丸生殖细胞肿瘤中p27蛋白阳性表达率与正常睾丸组织和慢性睾丸炎组织相比明显降低,差异具有统计学意义(P<0.05);在不同组织学亚型的睾丸生殖细胞肿瘤中的表达差异无统计学意义(P>0.05);在不同临床分期的睾丸生殖细胞肿瘤中的表达差异具有统计学意义(P<0.05)。结论:p27蛋白在临床分期高的睾丸生殖细胞肿瘤中的低表达,可为睾丸生殖细胞肿瘤的临床分期及预后情况提供有用的信息。

Objective： To detect the differences of p27 expression among human testicular germ cell tumors, normal testicle and chronic orchitls. Methods： p27 expression were evaluated in the tissues of 55 human testicular germ cell tumors and 10 normal testicular, 10 chronic orchitis using immunohistochemistry （S-Pmethod）. Results： Among the 55 testicular germ cell tumors, 26 cases showed positive staining of the p27 ,positive rate 47.3% , and 9 cases showed positive staining of the p27 in 10 normal testicular, positive rate 90. 0%, 8 cases showed positive staining of the p27 in chronic orchitis, positive rate 80. 0%. There was a significant difference of the p27 expression between testicular germ cell tumor and normal testicular or chronic orchitis （P 〈 0.05 ）, and there was not significant difference of the p27 expression in the testicular germ cell tumors of different histological subtype（ P 〉 0. 05 ） ,the positive expression rate of p27 was significantly decreased in higher clinical stage of testicular germ cell tumors （ P 〈 0.05 ）. Conclusion： Lower expression of P27 in higher clinical stage of testicular germ cell tumors provides valuable information for the clinical staging and prognosis of human testicular germ cell tumors.