摘要
A high-performance liquid chromatography method for the simultaneous determination of seven bioactive ginsenosides with diol stationary phase and isocratic elution was used to determin the ginsenosides in ginseng products. The optimization of the chromatographic separation was performed and the effect of temperature on separation was investigated. Using the validated procedure, the developed method was demonstrated to be more sensitive and effective than the conventional reversed-phase chromatography, where the chromatographic run is time-consuming to analyze a large number of ginsenosides. The results indicated that the developed method can be used for the quantitative determination of ginsenosides in complex ginseng samples.
本文描述了一种使用二醇柱在等度条件下同时测定人参样品中七种活性人参皂苷的高效液相色谱方法。通过评价温度对分离性能的影响优化了色谱条件，使用药物分析的评价方法，结果表明：与传统的耗时的C18固定相分离相比，此法更加灵敏和高效。实验结果也表明了此法在复杂人参样品中人参皂苷的定量测定中具有潜在的能力。
基金
supported by the postdoctoral fellowship of Jilin Institute of Chemical Technology
