几种不同高分子生物材料吸附FG与单克隆抗体M1结合亲合力的研究

FIBRINOGEN ADSORBED TO DIFFERENT POLYMER BIOMATERIALS AND STUDYING ON MONOCLONAL ANTIBODY M1 BINDING AFFINITY

利用鼠抗人血纤维蛋白原（Ｆｉｂｒｉｎｏｇｅｎ，ＦＧ）单克隆抗体Ｍ１作为血小板替代物，以酶联免疫吸附测定（ＥｎｚｙｍｅＬｉｎｋｅｄｌｍｍｕｓｏｒｂｅｎｔＡｓｓａｙ，ＥＬＩＳＡ）为手段，通过ＦＧ－Ｍ１复合物结合亲合力常数ｋａ的测定，对几种高聚物材料血液相容性进行了评价．测定了不同材料表面的ＸＰＳ谱。

Using monoclonal antibody M1 as a substitute of the platelet the binding affinity of FG M1 complex of a few polymer biomaterials was measured by ELISA.Compared the changs in binding affinity of M1 to one of the platelet binding domain of FG adsorbed to different materials so that evaluated the blood compatibility of those polymer biomaterials.