黄精凝集素Ⅱ对大鼠主动脉Ca^(2+)跨膜流动的影响

THE EFFECT OF POLYGONATUM CYRTONEMA HUA. LECTIN Ⅱ(PCL Ⅱ) ON CALCIUM TRANSMEMBRANE INFLUX INDUCED BY NOREPINEPHRINE AND KCl IN SMOOTH MUSCLE CELL OF RAT AORTA

用４５Ｃａ跨膜流动技术测定黄精凝集素Ⅱ对大鼠主动脉Ｃａ２＋跨膜内流的影响．黄精凝集素Ⅱ在０．１～１０μｍｏｌＬ－１时，不影响静息状态下Ｃａ２＋的内流，但能抑制ＮＥ和ＫＣｌ引起的Ｃａ２＋内流，其抑制能力依赖于凝集素的浓度；在１０μｍｏｌＬ－１时，可完全阻滞ＮＥ引起的Ｃａ２＋增加，并能有效地阻滞ＫＣｌ引起的内流作用．

Using 45 Ca as a tracer,the influence of Polygonatum cyrtonema Hua. (a Chinese herbal drug) Lectin Ⅱ(PCL Ⅱ) on Ca 2+ transmemrane influx in smooth muscle cell of mouse aorta was studied.PCL Ⅱ can block the potential dependent Ca 2+ channel (PDC) and receptor operated Ca 2+ channels(ROC) in cell membrane of smooth muscle effectively,the elevation of Ca 2+ induced by 1.2μmol·L -1 norepinephrine and 100mmol·L -1 KCl can be blocked effectively by PCL Ⅱ at the concentration of 10μmol·L -1 .As a carbohydrate recognization and binding proteine,PCL Ⅱ can be utilized as calcium channels blocker(CCB,calcium antagonist CA).It’s the first report about CCB(CA) can be from bio macromolecular,especially from phytohemagglutinin.