摘要
通过对酒精酵母进行紫外诱变及2,3,5-氯化三苯基四氮唑(TTC)的鉴别培养,获得了10株呈红色菌落表型的突变株。诱变条件为:紫外(灯管功率20W)波长254nm,照射距离20cm,照射时间10s、60s和80s,获得正突变菌株Z8,可以耐受12%vol酒精。同时表明了酒精高产和酒精耐性有一定的相关性。
Ten mutant strains of Saccharomyces cerevisiae showing red colony phenotype on the triphenyltetrazolium chloride medium were obtained in this paper. The mutation condition of ultraviolet lights were as followed： light strength 20 W, vertical distance 20 cm, wave length 254 nm and irradiation time 10 s, 60 s and 80 s, respectively. The strain Z8 tolerating 12%vol ethanol was obtained. The results also indicated that ethanol production was positively related with ethanol tolerance.
出处
《中国酿造》
CAS
北大核心
2010年第10期35-38,共4页
China Brewing
基金
海南省教育厅科研基金资助项目(Hj2009-22)
关键词
紫外诱变
酿酒酵母
酒精耐性
发酵
ultraviolet mutation
Saccharomyces cerevisae
ethanol tolerance
fermentation
