呋塞米-Pd(Ⅱ)-碱性三苯甲烷染料反应体系的吸收光谱及分析应用

Absorption Spectra and Applications of FUR-Pd (Ⅱ)-Triphenylmethane Dye Systems

在pH为5.0～7.6的Britton-Robinson(BR)缓冲溶液中,呋塞米(FUR)与Pd(Ⅱ)形成摩尔比1:1的配合物,进一步与乙基紫(EV)、结晶紫(CV)、甲基紫(MV)、亮绿(BG)、甲基绿(MeG)等碱性三苯甲烷染料(BTPMD)作用形成1:1的离子缔合物时,染料发生褪色反应,褪色波长分别位于595nm(EV、CV体系)、580nm(MV体系)、615nm(BG体系)和630nm(MeG体系),FUR浓度在2.0×10-7～4.0×10-6g/mL(EV体系)、3.0×10-7～8.0×10-6g/mL(CV体系)、4.0×10-7～4.0×10-6g/mL(MV体系)、4.0×10-7～7.0×10-6g/mL(BG体系)、1.2×10-6～8.0×10-6g/mL(MeG体系)范围内与褪色波长处的吸光度变化值呈良好的线性关系,摩尔吸光系数(ε)根据染料的不同在0.57×104～3.40×104L/(mol·cm)之间,灵敏度最高的EV体系的检出限(3σ)为6.0×10-8g/mL,据此建立一种测定呋塞米的新分光光度法。研究了适宜的反应条件、分析化学性质和共存物质的影响,用于尿样中呋塞米的含量测定,回收率在96.0%～106.8%之间。

In a pH 5.0 ～ 7.6 Britton-Robinson（BR） buffer medium,furosemide（FUR） reacted with Pd （Ⅱ） to form a 1：1 anionic chelate,which further reacted with some basic triphenylmethane dyes（BTPMD） such as ethyl violet（EV）,crystal violet（CV）,methyl violet（MV）,methyl green（MeG）,and brilliant green （BG） to form 1：1 ionic complexes.The absorption spectra of the dyes were changed and fading peak are 595 nm for EV and CV systems,580 nm for MV system,615 nm for BG system and 630 nm for MeG system.There was an linearity between the concentrations of FUR and ΔA of the solutions at the maximum fading peaks.The quantitative determination ranges of five the triphenylmethane dyes were 2.0 × 10-7 ～ 4.0 × 10-6 g/mL for EV system,3.0 × 10-7 ～ 8.0 × 10-6 g/mL for CV system,4.0 × 10-7 ～ 4.0 × 10-6 g/mL for MV system,4.0 × 10-7 ～ 7.0 × 10-6 g/mL for BG system and 1.2 × 10-6 ～ 8.0 × 10-6 g/mL for MeG system.Depending on the different dyes systems,the molar absorption coefficiencies（ε） of different systems were between 0.57 × 104 ～ 3.40 × 104 L/（mol·cm） and the detection limit （3ε） was 6.0 × 10-8 g/mL.A new spectrophotometry for the determination of FUR was set up based on above results.In the five systems,the optimal conditions of the reactions and the effects of external substances were investigated,the method has been applied to the determination of FUR in urine samples with satisfactory results.The mean recoveries ranged from 96.0% to 106.8%.