摘要
采用国产JQ－700型基因枪，以含Bt基因的质粒pHB101为供体，轰击小麦、玉米愈伤组织，轰击后的愈伤组织经潮霉素抗性筛选后，获得32株小麦再生苗，14株玉米抗性植株。以a－32P－dCTP标记的PHB101质粒为探针，对小麦抗性植株进行点杂交印渍检测，发现有15株小苗显示阳性信号，且其中有一株的后代对田间蚜虫及锈病的抗性较好。玉米再生植株种植田间后在其后代中选出一抗玉米螟的稳定株系，尚需进一步的分子验证和饲虫实验。
Calli of wheat and maize were subjected to JQ-700(made in China)gene-gun bombardment,using plasmid pHB101 containing Bt-gene as donor.32 regenerated wheat seedlings and 14maize resistant plants were obtained by Hygromyain resistance selecting .15 regenerated wheat resistant seedlings were confirmed to show positive signal by dot hybridization blotting test,using pHB101 plasmid labeled by a-32p-dCTP as probe.And the progeny of one plant appeared good resistance to aphid and rust in the field. One stable line resistant to corn borer had been selected in the progeny of maize regenerated plants, further molecular test and feeding-insect test are going on.
出处
《山东农业科学》
1999年第2期5-8,共4页
Shandong Agricultural Sciences
