鹿药组织培养的研究

Study of Tissue Culture of Smilacina laponica

为保护鹿药这种野生植物资源,以生长旺盛的鹿药根茎为材料,进行愈伤组织诱导、愈伤组织分化、试管苗的生根、扦插和移植的研究,成功地建立起鹿药根茎的再生体系。结果证明:MS+ZT0.2mg/L+CH20mg/L+2,4-D1.5mg/L(或2.0mg/L)是鹿药根茎愈伤组织诱导培养的理想培养基;MS+AgNO_30.2mg/L+BA0.2mg/L+KT0.3mg/L+NAA0.1mg/L是鹿药愈伤组织分化培养的理想培养基;1/2MS+BA0.4mg/L+NAA0.1mg/L是鹿药愈伤组织分化不定芽继代培养和增殖培养的理想培养基;1/3MS+NAA0.1mg/L+IAA0.4mg/L是鹿药生根培养和生根继代培养的理想培养基;移植到山坡上试管苗生长旺盛。

For the protection of wild resources, the exptants from strong growth Smilacina laponica stem segment were cultured to in- vestigate the effects of different external hormones on induction and differentiation of callus, formation of adventitious roots, cutting and transplantation. The asexual line of Smilacina laponica was MS＋ZT established successfully. The results show that the ideal medium for induction and differentiation of callus were MS＋ZT0.2mg/L＋CH20mg/L＋2, 4-D1.5mg/L（or 2.0mg/L）and MS＋AgNO30.2mg/L＋ BA0.2mg/L＋KT0.3mg/L＋NAA0.1mg/L respectively, the ideal differentiation and multiplication medium for adventitious buds was 1/ 2MS＋BA0.4mg/L＋NAA0.1mg/L, the ideal medium for rooting was 1/3MS＋NAA0.1mg/L＋IAA0.4mg/L. The tube seedlings transplanted to the hill grew orderly and flourishly.