高效液相色谱法测定防感喷雾剂中连翘苷的含量

Determination of Forsythin in Fanggan Spray with High Performance Liquid Chromatography

目的:建立防感喷雾剂中连翘苷的含量测定方法。方法:采用高效液相色谱法,选用AgilentC18色谱柱(250mm×4.6mm,5μm);流动相为乙腈-水(24∶76);检测波长277nm;流速1.0mL/min;进样量10μL;柱温30℃。结果:连翘苷在0.208～0.728mg/mL范围内线性关系良好,回归方程为Y=619397X-75609(r=0.99995,n=6),平均加样回收率为100.18%,RSD为1.53%。结论:本法简便快捷、灵敏,结果准确,可用于防感喷雾剂中连翘苷的质量控制。

Objective：To develop a method for determination of forsythin in Fanggan Spray. Methods：High performance liquid chromatography （HPLC） method was used. The separation was performed on an Agilent Cl8 （250 mm×4.6 mm,5 μm） column. The mobile phase was acetonitrile-water （24∶76）. The detection wavelength was 277 nm. The flow rate was 1.0 mL/min and the injection volume was 10 μL and the column temperature was 30 ℃. Results：The forsythin had a good linear relation within the range of 0.208～0.728 mg/mL （r=0.9999,n=6）. The average recovery was 100.18% and RSD was 1.53%. Conclusions：This method is simple,rapid,sensitive and accurate,and can be used as a method of quality control for forsythin in Fanggan Spray.