一起引起食物中毒副溶血性弧菌的检测

Survey of a food poisoning event caused by Vibrio parahaemolyticus

2008年7月杭州市下城区某员工食堂暴发一起由副溶血性弧菌(VP)引起的食物中毒事件,采集中毒患者粪便标本、厨师肛拭子标本和食物加工环节涂抹物标本共25份。经常规鉴定,共检出11株VP(10株来自患者粪便、1株来自食物加工环节涂抹物)。对11株VP进行血清分型和耐药性检测发现,11株VP血清型均为O3:K6型;神奈川溶血试验(KP)阳性;生物学性状和药敏试验结果均一致。采用聚合酶链反应(PCR)方法对11株菌进行种属特异基因(R72H)和毒力基因(tdh、trh)检测,同时应用脉冲场凝胶电泳技术(PFGE)进行分型和聚类分析,11株菌的VP种属特异基因(R72H)均为阳性,毒力基因复合PCR结果,tdh(434bp)阳性,trh(250bp)阴性;11菌株经PFGE聚类分析得到3种带型,聚类分析相似性百分比为93%。

In July 2008,a food poisoning event caused by Vibrio parahaemolyticus （VP） occurred in an employee canteen in Xiacheng district of Hangzhou. Twenty five samples were collected,which included patients＇ stool samples,cooks＇ rectal swabs and processing smear samples. By routine detection,eleven strains of VP were identified （10 from patients＇ stool samples and 1 from smear sample）. The serotying and drug susceptibility test indicated that 11 strains of VP belonged to O3：K6 serotype and were positive to kanagawa phenomenon （KP）. The biological characters and the results of drug susceptibility test were consistent among 11strains of VP. The polymerase chain reaction （PCR） was used genus-specific gene （ R72H） and virulence genes （ tdh and trh）. The subtyping and cluster analysis were conducted by using pulsed field gel electrophoresis （PFGE）. R72H was detected in all 11 strains. Multiplex PCR indicated that the strains were positive for tdh （434bp） and negative for trh （250bp）. The 11 strains had 3 PFEG patterns. The cluster analysis indicated that the similarity was 93%.