摘要
纯化的减蛋综合征病毒(EDSV)DNA经HindⅢ水解、0.8%琼脂糖电泳后,回收各DNA条带并克隆至pBluescriptKS载体,建立了EDSV基因文库,对33K蛋白基因进行了分析。本研究证实,EDSV33K蛋白基因含有2个外显子,与羊腺病毒(OAV)33K蛋白比较,N端编码产物的同源性为30.8%,C端的为60%。用RT-PCR扩增33KmRNA和cDNA克隆及序列分析证实,EDSV33K蛋白含有82碱基(nt)的内含子,去掉内含子33K蛋白基因能形成完整的ORF,其编码产物由177氨基酸(aa)组成,推测分子质量为2.06×104,与人5型腺病毒和OAV的同源性分别为28.1%和44.7%。
Egg drop syndrome virus(EDSV), AA2 strain isolated in China, was purified and the viral DNA was digested by restriction enzyme Hind . The fragments were cloned into the sites of pBluescript KS(+) vector respectively. The whole genome library was constructed and the sequence of Hind E fragment was determined. The fragment is 3 209 nt in length, encoding C terminal of 100K, 33K, p VIII and Nterminal fiber protein. The EDSV 33K protein gene is interrupted by an intron. The homology of the Nterminal and Cterminal exon of 33K with that of ovine adenovirus (OAV) is 30.8% and 60% respectively. The cDNA sequence analysis shows the 33K intron is 82 nt in length. The predicted 33K protein consists of 177aa with 2.06104 in molecular weight. The amino acid sequence has a homology of 28.1% and 44.7% to that of Ad5 and OAV respectively.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1999年第3期221-225,共5页
Chinese Journal of Veterinary Science
基金
国家自然科学基金
农业部重点资助