克隆伊氏锥虫2个VSG的分离纯化和电泳分析

Isolation and Electrophoresis Analysis on Two VSG of a Cloned Trypanosoma evansi

用蛋白酶抑制剂ＴＬＣＫ对伊氏锥虫安徽株单虫克隆的２个抗原变异体ＳｈＴａｔ１．３和ＳｈＴａｔ１．５的变异表面糖蛋白（ＶＳＧ）进行了分离纯化，采用ＳＤＳ－ＰＡＧＥ和等电聚焦电泳对ＶＳＧ的分子量和等电点进行比较研究。结果，ＶＳＧ的分子量约为４００００，等电点约为ｐＨ５．０，但２个抗原变异体的ＶＳＧ在分子量和等电点都有差异，提示其抗原变异由ＶＳＧ变化引起。

Two VSG of T evansi were isolated and purificated by applying proteolytic enzyme inhititor TLCK. Molecular weight of VSG is about 40 000 measured by SDSPAGE and isoelectric point about pH5.0 by isoelectrofocusing. VSG of ShTat1.3 and ShTat1.5 were difference either in molecular weight or in isoelectric point. The results prove antigenic variation of T evansi is caused by change of VSG.