白细胞介素-1α对仔猪睾丸间质细胞睾酮分泌的影响

Effects of Interleukin1 (IL1) on the Testosterone Secretions in Piglet Leydigs Cell

用体外培养的仔猪睾丸间质细胞，研究了白细胞介素－１α（ＩＬ－１α）对间质细胞睾酮分泌的影响。结果，ＩＬ－１α能抑制ｈＣＧ诱导的间质细胞分泌睾酮，其睾酮产生量随ＩＬ－１α剂量的增加及作用时间的延长而下降；用２０Ｕ／ｍＬ和５０Ｕ／ｍＬ的ＩＬ－１α作用４８ｈ，可分别抑制６０％及６５％的睾酮分泌量；但无ｈＣＧ诱导时，５０Ｕ／ｍＬ的ＩＬ－１α可使间质细胞基础睾酮的生成量增加１倍。提示睾丸内ＩＬ－１α可能通过多种途径调节间质细胞睾酮的产生。

The experiment studied the effect of IL1 on testosterone secreted in Leydigs cell by culturing in vitro. When Leydigs cells were cultured with 20, 50 U/mL of IL1 for 48 h and then were stimulated with 50 U/mL of hCG for 4 h, 60% and 70% of hCGinduced testosterone production were inhibited respectively. And 50 U/mL of IL1 increased by 100% the production of basal testosterone in Leydigs cell. The result suggests that IL1, as a potential local factor, affects the testosterone production in Leydigs cell.