紫外分光光度法测定盐穗木生物碱含量

Determination on the contents of alkaloid in Halostachyscaspica with ultraviolet spectrophotometry

探讨盐穗木中有效成分生物碱含量测定方法,用紫外分光光度法测定盐穗木中生物碱的含量,选定波长为280 nm。结果显示,测定在0.079-0.573 mg/mL范围内线性关系良好,回归方程为A=69.20 C+0.019,R=0.999(n=5)。采用紫外分光光度法测定盐穗木中生物碱含量具有精度高、稳定性好、操作简便的特点,是提取盐穗木全草生物碱的有效测定方法。

Ultraviolet spectrophotometry was used to determine the contents of alkaloid in Halostachyscaspica.The detecting wavelength was 280 nm.The results showed a good linear relationship within the range of 0.079～0.573 mg/mL,the regression equation was A = 69.20 C ＋0.019,R^2 = 0.999（n = 5）.It is an effective way to determine the contents of alkaloid in Halostachyscaspica with high accuracy,well stability and simpleness.