高效液相色谱法测定芴酮溴代产物中2,7-二溴芴酮含量

HPLC Determination of 2,7-Dibromofluorenone in Bromo-derivatives of Fluorenone

提出了采用高效液相色谱测定芴酮溴代产物中2,7-二溴芴酮含量的方法。采用EliteHypersil ODS2 C18色谱柱（4.6 mm×250 mm,5μm）作固定相,以甲醇与水体积比为85比15的混合液为流动相,流量为1.0 mL·min^-1,采用波长为254 nm的紫外检测器进行检测。2,7-二溴芴酮质量浓度在5.0-40.0 mg·L^-1范围与其峰面积呈线性关系。在3个浓度水平上（即5.00,10.00,15.00 mg·L^-1）对方法的回收率进行测定,测得结果在98.2%-99.1%之间,各测定值的相对标准偏差（n=6）均小于1%。

HPLC was applied to the determination 2,7-dibromofluorenone in bromo-substituted fluorenones.Elite Hypersil ODS2 C18 column（4.6 mm×250 mm,5 μm） was used as the stationary phase,and a mixture of methanol and water（mixed in the ratio of 85 to 15 by vol.） was used as the mobile phase with flow-rate of 1.0 mL·min-1.UV detection at 254 nm was adopted in the determination.Linear relationship between values of peak area and mass concentration of 2,7-dibromofluorenone was obtained in the range from 5.0 to 40.0 mg·L^-1.Tests for recovery were performed by addition of standard solution of 2,7-dibromofluorenone at 3 concentration levels（i.e.5.00,10.00 and 15.00 mg·L^-1）,values of recovery found were ranged from 98.2% to 99.1%.Values of RSD′s（n=6） found from the analytical results were all less than 1%.