LC-MS/MS法和mFPIA法测定全血环孢素A浓度的相关性比较

Correlations of LC-MS/MS and mFPIA for the Determination of Whole Blood Cyclosporin A Concentration

目的:对高效液相色谱电喷雾串联质谱(LC-MS/MS)法和单克隆荧光偏振免疫(mFPIA)法测定肾移植术后稳定期患者不同时间点的全血环孢素A(CsA)浓度进行相关性比较。方法:分别采用LC-MS/MS法和mFPIA法测定患者全血CsA浓度,对测定结果进行双侧配对t检验比较差异性,用Passing-Bablok回归分析法比较不同采血时间点2种方法测定结果的相关性。结果:2种方法测定值之间具有显著性差异;服药后0、3.0、4.0h时2法测定值的相关性最好,0.5、0.75、12.0h相关性最差。结论:对CsA血药浓度监测结果的分析需考虑监测方法以及不同采血时间点的影响。

OBJECTIVE：To compare the correlations of LC-MS/MS and mFPIA for the determination of the whole blood Cyclosporin A（CsA）concentration in patients after renal transplantation in stable phase at different time.METHODS：The concentrations of CsA in patients were assayed by LC-MS/MS and mFPIA respectively.The difference of results was analyzed by the two-sided paired t-test and the correlation between the results of 2 kinds of methods at different time were detected by Passing-Bablok regression analysis.RESULTS：There was significant difference between results of 2 kinds of methods;obvious correlation was found between results of 2 kinds of methods at 0 h,3.0 h,4.0 h after administration and no obvious correlation was found at 0.5 h,0.75 h,12.0 h.CONCLUSION：Effect of monitoring method and different sampling time should be taken into account in monitoring analysis of blood concentration of CsA.