母血羊水中IL-6和IL-8水平与绒毛膜羊膜炎关系的研究

Relationship between IL-6 and IL-8 Levels in Maternal Blood and Amniotic Fluid and Chorial Amnionitis

目的探讨白细胞介素－６（ＩＬ－６）和白细胞介素－８（ＩＬ－８）在监测胎膜早破中的作用。方法采用酶联免疫吸附实验对４６例胎盘早破孕妇母血清、羊水中ＩＬ－６和ＩＬ－８水平进行监测，并以正常足月妊娠孕妇２０例做对照组。结果胎膜早破孕妇母血清ＩＬ－６、ＩＬ－８和羊水中ＩＬ－６和ＩＬ－８水平均较正常足月妊娠组高，差异显著（Ｐ＜００１，Ｐ＜００５）；随着破膜时间延长母血ＩＬ－６、ＩＬ－８和羊水中ＩＬ－６、ＩＬ－８有增加趋势；绒毛膜羊膜炎患者１３例，其母血、羊水中ＩＬ－６和ＩＬ－８水平均明显高于非绒毛膜羊膜炎患者（Ｐ＜００５）。结论测定胎膜早破孕妇羊水、血中ＩＬ－６和ＩＬ－８水平对识别绒毛膜羊膜炎起辅助诊断作用。

Objective\ To explore the effect of interleukin-6(IL-6) and interleukin-8(IL-8)in monitoring premature rupture of fetal membranes.Methods\ Using enzyme linked immunosorbent assay(ELISA),IL-6 and IL-8 levels in maternal blood and ammiotic fluid were detected(monitored)in 46 cases of premature rupture of fetal membranes,20 normal full-termed pregnant women as controls.Results\ In premature rupture of fetal membranes group,IL-6 and IL-8 levels in maternal blood and in amniotic fluid were significantly higher than in normal full-termed pregnant women group( P <0 01, P <0 05).IL-6 and IL-8 levels in maternal blood and in amniotic fluid showed a tendency of increasing with time prolongation after rupture of membranes.In 13 cases of chorial amnionitis,the IL-6 and IL-8 levels in maternal blood and amniotic fluid were dramatically higher than those in patients without chorial amnionitis( P <0 05).Conclusions\ Detection of IL-6 and IL-8 levels in maternal blood and amniotic fluid in patients with premature rupture of fetal membranes could play an important role in accessory diagnosis of chorial amnionitis.