摘要
应用ＧＰＰＣＲ 技术对４０ 例宫颈癌活检组织进行ＨＰＶ 感染的检测，发现有３５ 例为阳性（ 阳性率为８７％ ），同时利用ＰＣＲＳＳＣＰ（单纯ＤＮＡ构象多态性）分析技术，对３５ 例ＨＰＶ 阳性的宫颈癌组织进行Ｐ５３ 基因５ ～６外显子的检测，其突变率仅为２－８５ ％（１／３５），而在５ 例ＨＰＶ 阴性的宫颈癌组织中未检测到Ｐ５３ 基因突变，结果表明：Ｐ５３ 基因的突变在宫颈癌的发生过程中不起主要作用，并且与ＨＰＶ的感染无相关性。
We applied GP PCR(general primers PCR) to detecting HPV infection in 40 human cervical carcinoma.The postive rate was 87%(35/40).P53 gene 5～6 exon was detected in 35 HPV infection biopsytissues by PCR SSCP analysis.The mutation rate was only 2.85%(1/35).But P53 gene mutation was not detected in 5 human cervical carcinoma without HPV infection.The findings show P53 gene mutation doesn't play an important role in human cervical carcinoma and has no correlation with HPV infection.
