喉癌组织中P16蛋白表达的检测及临床相关性研究

Examination of P16 protein expression in human laryngeal carcinoma and study of its clinical correlation

目的：探讨喉鳞状细胞癌中Ｐ１６蛋白表达的情况及临床相关性。方法：用免疫组化的方法检测４２例喉鳞癌组织中Ｐ１６蛋白的表达情况。结果：Ｐ１６蛋白表达的阳性率为４７．６２％（２０／４２）。其中声门上型组为４０．００％（４／１０），声门型组为５７．８９％（１１／１９），声门下型组为３８．４６％（５／１３）；细胞分化Ⅰ级组为７５．００％（１２／１６），Ⅱ～Ⅲ级组为３０．７７％（８／２６）；伴有颈淋巴结转移组为２０．００％（３／１５），无颈淋巴结转移组为６２．９６％（１７／２７）；临床Ⅰ期组为４０．００％（８／２０），Ⅱ～Ⅳ期组为５４．５４％（１２／２２）。结论：提示Ｐ１６蛋白的表达与癌细胞的分化程度及肿瘤的转移情况有关，与临床分型及分期无关。

Objective:To study the expression of P16 gene and its correlation with clinical cases. Methods:The levels of P16 gene products were examined immunochemically in resected specimens of human laryngeal squamous cell carcinoma. Results: Carcinomas tested: 47.62%(20/42) showed positive staining in the lesions. 40.00% (4/10) in supraglottic cancer group, 57.89% (11/19) in glottic cancer group, 38 46% (5/13) in subglottic cancer group, 75.00% (12/16) in cell differentiation gradeⅠgroup, 30.77% (8/26) in cell differentiation grade Ⅱ￣Ⅲ group, 20.00% (3/15) in lymph node metastasis group, 62 96% (17/27) in those without metastasis, 40.00 %(8/20) in clinical stageⅠgroup, 54.54% (12/22) in stageⅡ￣Ⅳgroup. Conclusion:The expression of P16 protein is related with malignant degree and lymph metastasis in human laryngeal carcinoma.