移植NT-4基因修饰细胞对大鼠前角运动神经元损伤的保护作用

The protective effect of grafting NT 4 genetically modified cells on injury of spine motoneurons in rats

目的：观察神经营养素４（ＮＴ－４）基因修饰细胞对成年大鼠脊髓前角运动神经元损伤的保护作用。方法：应用逆转录病毒介导的体外ＮＴ－４基因转移方法，制备高表达ＮＴ－４的基因修饰细胞。离断大鼠左侧坐骨神经作为外周神经损伤模型，右侧作为对照，在离断处移植ＮＴ－４基因修饰细胞。观察大鼠脊髓前角运动神经元尼氏染色和胆碱酯酶染色的变化。结果：在移植ＮＴ－４基因修饰细胞组和对照组间，尼氏和胆碱酯酶染色有显著性差异。结论：移植ＮＴ－４基因修饰细胞对外周神经损伤所造成的运动神经元的退变有显著的改善作用。

Objective: To study the protective effect of grafting NT 4 genetically modified cells on injury of spine motoneurons in rats. Methods: NT 4 genetically modified cells were obtained by infecting L 6TG cell in vitro with a retroviral vector pN 2A containing the rat NT 4 cDNA gene. The rats with left sciatic nerve apocoped were taken as model for treatment by implanting NT 4 genetically modified cells. The right sciatic nerve was taken as control. The Nissl staining and ChE staining in motoneurons of spine were also studied. Results: There were significant differences between groups of NT 4 and control in the Nissl staining and ChE staining. Conclusion: Grafting NT 4 cells can prevent most motoneurons from death.