期刊文献+

Northern印迹法的改进 被引量:2

下载PDF
导出
摘要 Alwine发明的Northern印迹法被广泛用于检测mRNA的大小和丰度,但由于其降解酶RNase广泛存在,且理化性质稳定,故在实际操作中,尤其是从组织中提取总RNA进行Northern印迹,对那些设备不是很完善的实验室来说,得到一个比较理想的杂交...
作者 郭兴中
出处 《第二军医大学学报》 CAS CSCD 北大核心 1999年第3期195-196,共2页 Academic Journal of Second Military Medical University
基金 国家自然科学基金
  • 相关文献

同被引文献18

  • 1徐佳,刘志锋,姜勇.高迁移率族蛋白与真核基因表达调控[J].生物化学与生物物理进展,2005,32(5):397-402. 被引量:19
  • 2STEMMER C,SCHWANDER A,BAUW G,et al.Protein kinase CK2 differentially phosphorylates maize chromosomal high mobility group B (HMGB) proteins modulating their stability and DNA interactions[J].The Journal of Biological Chemistry,2002,277(2):1092-1098.
  • 3GRASSER KD.Chromatin-associated HMGA and HMGB proteins:versatile co-regulators of DNA-dependent processes[J].Plant Mol Biol,2003,53:281-295.
  • 4GRASSER KD,GRILL S,DUROUX M,et al.HMGB6 from Arabidopsis thaliana specifies a novel type of plant chromosomal HMGB protein[J].Biochemistry,2004,43(5):1309-1314.
  • 5ZHENG CC,BUI AQ,O'NEILL SD.Abundance of an mRNA encoding a high mobility group DNA-binding protein is regulated by light and an endogenous rhythm[J].Plant Mol Biol,1993,23(4):813-823.
  • 6O'NEILL SD,ZHENG CC.Abundance of mRNA encoding HMG1/HMG2 class high-mobility-group DNA-binding proteins are differently regulated in cotyledons of Pharbitis nil[J].Plant Mol Biol,1998,37:235-241.
  • 7KWAK KJ,KIM JY,KIM YO,et al.Characterization of transgenic Arabidopsis plants overexpressing high mobility group B proteins under high salinity,drought or cold stress[J].Plant Cell Physiol,2007,48(2):221-231.
  • 8BUSTIN M.Regulation of DNA-dependent activities by the functional motifs of the high-mobility-group chromosomal proteins[J].Mol Cell Biol,1999,19(8):5237-5246.
  • 9GOODWIN GH,SANDERS C,JOHNS EW.A new group of chromatin associated proteins with a high content of acidic and basic amino acids[J].Eur J Biochem,1973,38(1):14-19.
  • 10BUSTIN M.Revised nomenclature for high mobility group (HMG) chromosomal protein[J].Trends Biochem Sci,2001,26(3):152-153.

引证文献2

二级引证文献5

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部