β-榄香烯诱导人肝癌细胞株SMMC-7721凋亡的研究

Elemene induced apoptosis of SMMC 7721 cells of human hepatocellular carcinoma

目的：探索榄香烯对体外培养人肝细胞株的作用及其机制。方法：应用ＭＴＴ方法分析榄香烯对肝癌细胞株ＳＭＭＣ－７７２１生长的影响。结果：榄香烯能明显抑制肝癌细胞生长，其半数生长抑制剂量为３７．４μｇ／ｍｌ；流式细胞术证实榄香烯能阻滞肝癌细胞从Ｇ０／Ｇ１期进入Ｓ期，并诱发细胞凋亡；透射电镜超微结构证实榄香烯能诱发肿瘤细胞凋亡的典型形态变化；免疫组化ＳＰ法及流式细胞术定量分析榄香烯能使肝癌细胞癌基因ｂｃｌ－２、ｃ－ｍｙｃ表达降低，抑癌基因ｐ５３表达增强。结论：榄香烯能诱导肝癌细胞凋亡，其作用机制可能与癌基因ｂｃｌ－２、ｃ－ｍｙｃ表达减少、ｐ５３表达增加有关。

Objcetive: To determine whether elemene can induce apoptosis of SMMC 7721 cells in vitro and to analyze the potential role of Bcl 2, C myc and P53 proteins in the regulation of the cellular apoptosis. Methods: SMMC 7721 cells were determined with MTT assay. Flow cytometry (FCM) analysis was carried out to quantify cellular apoptosis and the changes of cell cycle. Expression of Bcl 2, C myc and P53 proteins was detected with immunocytochemical technique and FCM. Results: Exposure of SMMC 7721 cells to elemene resulted in an inhibition of cell growth. The IC 50 value of elemene for SMMC 7721 cells was 37.4 μg/ml. The inhibition of elemene on the proliferation of SMMC 7721 cells consisted of cell cycle arrest in the transition from G 0/G 1 to S phase to induce apoptosis of the cells, which was confirmed by characteristic ultrastructural changes and by apo hypodipoid DNA peak on FCM. After 24 hours of exposure to elemene, FCM revealed that bcl 2 and c myc were significantly downregulated while p53 up regulated. Conclusion: Elemene might exert effects on SMMC 7721 cel line of human hepatocellular carcinoma through an inhibition on cellproliferation to induce apoptosis. The mechanism might be related to the combination of down regulation of bcl 2 and c myc and an up regulation of p53.