食管癌DCC基因和YNZ22位点串联重复序列的杂合性丢失

Loss of heterozygosity at YNZ22 and DCC loci containing variable number of tanden repeat seqment in esophageal carcinoma

目的：探讨抑癌基因杂合性丢失（ＬＯＨ）在食管癌发生发展中的作用。方法：应用聚合酶链反应技术对４６例食管癌ＹＮＺ２２位点和ＤＣＣ基因数目可变的串联重复序列（ＶＮＴＲ）区进行了分析。结果：食管癌ＹＮＺ２２位点ＬＯＨ率为４４．４％；ＤＣＣ基因为３２．１％。ＬＯＨ与组织学类型、肿瘤大小、浸润深度和淋巴结转移无明显相关。结论：ＹＮＺ２２位点和ＤＣＣ基因的ＬＯＨ参与了食管癌的发生发展。

Objective: To evaluate the role of loss of heterozygosity (LOH) at tumor suppressor genetic loci in the carcinogenesis of esophageal carcinoma. Methods: Polymorphic analysis of the genetic loci of YNZ22 and DCC containing a variable number of tandem repeat was performed in 46 surgically resected specimens of esophageal carcinoma with PCR. Results: LOH and YNZ22 and at DCC were detected in 44.4% and 32.1% of the cases of our series respectively. No significant correlation existed between the LOH at YNZ22 and DCC loci and the histological types, tumor size, systemic invasion and lymph node metastasis of the carcinoma ( P >0.05). Conclusion: Our findings suggest that LOH at YNZ22 and DCC genetic loci may be involved in the carcinogenesis of esophageal carcinoma.