丹参提取物F抗大鼠胃窦部粘膜再灌注损伤作用的研究

Effect of DSEF on reperfusion injury in gastric antrum

目的：探讨丹参提取物Ｆ（ＤＳＥＦ）抗大鼠胃窦部粘膜再灌注损伤的作用。方法：大鼠随机分为假手术组（ＳＣ）、单纯失血组（ＨＥ）、生理盐水组（ＮＳ）及丹参提取物Ｆ组（ＤＳＥＦ）；ＳＣ组大鼠除未行放血及血液回输外，行所有操作步骤，ＨＥ组大鼠经２０ｍｉｎ的失血性休克后处死，ＮＳ及ＤＳＥＦ组在休克２０ｍｉｎ后，回输全部血液，维持２０ｍｉｎ后处死。ＳＣ、ＨＥ、ＮＳ组静滴ＮＳ（０．０３ｍＬ／ｍｉｎ），ＤＳＥＦ组静滴ＤＳＥＦ（１ｇ／１００ｇ．ｗｔ）。结果：ＨＥ组损伤指数为０，ＮＳ组损伤指数明显高于ＤＳＥＦ组（Ｐ＜０．０１），且前者Ⅱ、Ⅲ级损伤占６０８４％，后者为３０３５％；ＮＳ组胃窦部粘膜细胞内钙含量明显高于ＳＣ组（Ｐ＜０．０１），ＤＳＥＦ组细胞内钙含量明显低于ＮＳ组（Ｐ＜０．０５）。结论：再灌注可致胃窦部粘膜损伤，ＤＳＥＦ具有抗胃窦部粘膜再灌注损伤的作用。

AIM:To study the effect of DSEF(Dan Shen Extract F) on reperfusion injury in gastric antrum. METHODS:Rats were randomly divided into group SC(rats underwent all procedures but hemorrhagic shock), HE(rats suffered from hemorrhagic shock for 20 minutes), NS and DSEF(rats received blood reperfusion after shock for 20 minutes and were killed after 20 minutes);Rats in group DSEF received intravenous infusion of DSEF(1 g/100 g BW), and normal saline was infused (0.03 mL/min) in the other groups. The index and depth of mucosal lesion and intracellular calcium ion concentration were measured. RESULTS:The index and depth in group DSEF were markedly lower than that in group NS( P <0.01), and the index in group HE was 0; As compared with that in group SC, the content of intracellular calcium in group NS increased significantly ( P <0.01), and intracellular calcium ion concentration in group DSEF was much lower than that in group NS( P <0.05). CONCLUSION:Reperfusion can cause gastric mucosal injury of antrum, which can be resisted by DSEF.