番茄斑萎病毒核衣壳蛋白基因的克隆与分析

Cloning and sequence analysis of the nucleoprotein genes of Tomato spotted wilt virus isolates from Yunnan

从云南表现褪绿黄化症状的番茄上分离到番茄斑萎病毒(Tomato spotted wilt virus)YN-1株系,利用RT-PCR方法克隆了该株系的核衣壳蛋白基因,并测定了该基因的序列,全长为777 bp,编码258个氨基酸。对该基因的氨基酸序列分析结果表明,YN-1 N基因与韩国3个株系的氨基酸序列相似性均高达97%。

Tomato spotted wilt virus （TSWV） was detected via RT-PCR from tomato with mottle symptom col- lected from Yunnan. The N gene, encoding nucleoprotein, was cloned and sequenced. Sequence and analysis results indicated that the N gene was 777 bp in length and encoded 258 amino acids. Sequence analyses showed N gene shared 99% and 97% nucleotide identities with the isolates from South Korea and Taiwan, respectively. In amino acid levels, the sequence identity was 99% and 98%, respectively.