支气管哮喘患者气道中白细胞介素-17表达与气道重塑关系的研究

Correlation between the expression of airway IL-17 and airway remolding in patients with bronchial asthma

目的初步探讨白细胞介素(IL)-17在支气管哮喘患者气道重塑中的作用。方法选取2007年9月至2008年9月门诊行气管镜检查的非急性发作期支气管哮喘患者40例,按肺功能测定结果分为2组。采用免疫组织化学方法分别检测其支气管肺泡灌洗液中肺泡巨噬细胞及气道上皮细胞的IL-17及转化生长因子(TGF)-β1的表达。采用方差分析和SNK-q检验进行统计学分析。结果支气管肺泡灌洗液肺泡巨噬细胞和气道上皮细胞中IL-17表达试验组分别为(0.361±0.190)和(0.306±0.014),较对照组[分别为(0.206±0.020)和(0.196±0.031)]明显升高,差异均有统计学意义(P<0.01);而TGF-β1表达试验组分别为(0.280±0.018)和(0.274±0.004),较对照组[分别为(0.206±0.006)和(0.193±0.015)]明显升高,差异均有统计学意义(P<0.01)。结论 IL-17可能在支气管哮喘气道重塑的发生发展中发挥作用。

Objective To investigate the role of IL-17 on airway remolding in patients with bronchial asthma.Methods 40 patients with non-episodic bronchial asthma who underwent bronchoscopy from September 2007 to September 2008 in the out-patient department were selected and divided into 2 groups（the experimental group and control group） according to the lung function test results.The levels of IL-17 and transforming growth factor-β1（TGFβ1） in the alveolar macrophages and airway epithelial cells from bronchial alveolar lavage fluid were detected using immunocytochemical method.Statistical analyses were performed using one-way ANOVA and SNK-q test.Results There was a significant increase in the level of IL-17 in alveolar macrophages and airway epithelial cells [（0.361 ± 0.190） and（0.306±0.014）,respectively] compared with the control group [（0.206±0.020） and（0.196±0.031）,respectively]（P〈0.01）.And there was a significant increase in the level of TGF-β1 in alveolar macrophages and airway epithelial cells [（0.280 ±0.018） and（0.274 ±0.004）,respectively] compared with the control group [（0.206 ±0.006） and（0.193±0.015）,respectively]（P〈0.01）.Conclusion IL-17 may play a role in the pathogenesis of airway remolding in bronchial asthma.