黄瓜性别控制基因CsACS1G的分子标记及其快速检测

Identification of CsACS1G gene DNA markers and DNA rapid detection in cucumber

根据黄瓜性别控制基因CsACS1G、CsACS1及BCAT基因DNA差异序列,设计CsACS1G基因特异标记引物G1/G2,采用碱处理法,快速提取黄瓜品种WI1983G、95、98-17、S-2-98及95×WI1983G的F1、F2群体植株DNA,并以此为模板进行PCR扩增.为了验证特异引物对检测CsACS1G基因的准确性,在黄瓜开花期间进行田间调查,结果表明,以快速提取的黄瓜DNA为PCR模板,特异引物G1/G2能稳定、准确扩增出CsACS1G基因特异片段,电泳检测结果与田间调查结果一致.

Based on the sequences of CsACS1G,CsACS1 and BCAT,the specific PCR primers were designed to identify CsACS1G gene using DNA markers in Cucumber （Cucumis sativus L.）,and genome DNA was rapidly extracted from cucumber WI1983G,95,98-17,S2-98 and offspring 95×WI1983G with the method of NaOH.Field surveys were conducted in the flowering period so as to confirm stability of the primers.The results showed that DNA molecular marker can amplify a specific fragment of CsACS1G gene when the resultant DNA was used as PCR template.So,identification of CsACS1G gene DNA molecular markers and the method of DNA rapid extraction in cucumber would provide the conditions to accelerating breeding of gynoecium＇s cucumber.