氟苯尼考在鸡粪中的降解特性

Degradation of florfenicol in chicken feces

为探明鸡粪中氟苯尼考的降解特性,采用气相色谱法,分别研究微生物、有机质、光照、氟苯尼考起始浓度与鸡粪中氟苯尼考降解的关系,以20℃恒温培养箱中避光培养的未灭菌、未去有机质的鸡粪中氟苯尼考降解为对照,结果表明,对照氟苯尼考的降解半衰期为1.9d,降解动力学方程为Ct=7.81×e-0.3699t,11d的降解率达95%;与对照相比,高温灭菌和去除有机质后,鸡粪中氟苯尼考的降解能力显著下降,半衰期分别提高到6.4、7.2d;光照处理与避光处理(对照)鸡粪中氟苯尼考降解的半衰期、降解速率常数和降解速率方程均无明显差异;氟苯尼考起始含量5、10mg/kg的半衰期差异不大,分别为1.8、1.9d,而20mg/kg的半衰期延长至5.6d.

In order to its degradation characteristics by use of GC,the relationship between micro-organisms,organic matter,light,initial concentration of florfenicol,and florfenicol in chicken feces were investigated,the degradation of florfenicol in non-sterilized chicken feces without organic matter removing was taken as the controlled group by culturing it in incubator with constant temperature at 20 ℃ under the dark.The result indicated that chicken feces was prevented from light under 20 ℃ condition,the half-life of florfenicol was 1.9 d,the degradation kinetic equation was Ct=7.81×e-0.369 9t,the degradation rate reached 90% within 11 d.Compared with the controled group,the degradation rate of florfenicol in chicken manure decreased markedly under the handling of sterilization and removal of organic matter.The half-lives grew up to 6.4 d and 7.2 d.The differences between the Initial concentration of 5 mg/kg and 10 mg/kg were not remarkable,and the half-life was 1.8 d and 1.9 d respectively,while when the Initial concentration rise to 20 mg/kg,the half-life extend to 5.8 d.Under either the condition of illumination or light prevention,the half-life,degradation kinetic equation and constant of degradation rate constant of florfenicol displayed no differences.