摘要
以徐薯18型甘薯茎叶为原料,建立了高速逆流色谱法制备分离甘薯茎叶中化合物的方法.以正己烷-乙酸乙酯-甲醇-水(0.5%乙酸)(体积比0.5:5:1:5)为溶剂系统,上相为固定相,下相为流动相,流速2mL/min,转速900r/min,进行2次分离,并结合1次制备高效液相,共分离得到7个高纯度化合物.结合化学显色反应、紫外扫描、高效液相色谱等,初步鉴定4个为黄酮类化合物,其中1个为芦丁,1个为槲皮苷.
Leaves of Xushu-18 was used as raw material to have established a method for isolation and preparation of chemical compositions from sweet potato stalk by high speed counter-current chromatography-high performance liquid chromatography （HSCCC-HPLC）.N-hexane-ethyl acetate-methanol-water（0.5% acetic acid） （0.5：5：1：5,V/V） was used as the two-phase solvent system.The upper one was used as the stationary phase,while the lower phase was used as the mobile one.Separation was performed twice at a flow-rate of 2 mL/min and a revolution speed of 900 r/min.Seven compounds were got and HSCCC was separated in the same solvent system and preparation high performance liquid chromatography.The structures were identified primarily by colour reaction,UV spectrometry and HPLC.The results showed one of them was rutin and the other was quercitrin.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第5期581-584,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省科学技术厅项目(2009NK3106)
湖南省教育厅项目(10C0799)
关键词
甘薯茎叶
高速逆流色谱
高效液相色谱
制备
sweet potato stalk and leaf
high speed counter-current chromatography
high performance liquid chromatography
isolation and preparation
