软肝抗癌止痛颗粒中延胡索乙素含量测定方法研究

Determination of the Content of Tetrahydropalmatine in Ruangankangaizhitong Granules by HPLC

目的:制定软肝抗癌止痛颗粒中延胡索乙素含量测定方法。方法:采用HPLC法,色谱柱为Agilent C18(150×4.6nm;5μm),流动相为甲醇-水-磷酸(70:30:1.2);检测波长280nm。结果:加样回收率为96.75%;RSD为1.59%;相关系数r=0.9999;五批软肝抗癌止痛颗粒中延胡索乙素范围为0.0995～0.109mg·g-1。结论:该方法准确、可靠、重复性好,可作为软肝抗癌止痛颗粒质量控制方法。

Objective：To establish a HPLC method to determine the content of Tetrahydropalmatine in Ruangankangaizhitong Granules.Methods：Select Agilent C18（150×4.6nm;5μm）;The mobile phase was consisted of methanol-water-phosphoric acid（70：30：1.2）;The detection wavelength was at 280nm;Results：The average recovery was 96.75%;RSD=1.59%;r=0.9999;The content of Tetrahydropalmatine ranged from 0.0995 to 0.109mg·g-1.Conclusion：This method is accurate,reliable and with good reproducibility,It can be used for the quality control of Ruangankangaizhitong Granules.