宁夏道地药材银柴胡种子生活力等指标的测定

Determination of Seed Viability of Stellaria dichotoma of Ningxia Genuine Traditional Drugs

目的:通过对种子生活力等指标的测定,为完善银柴胡种子检验标准操作规程提供实验依据。方法:采用2,3,5-氯化三苯四氮唑(TTC)染色法测定种子的活力。结果:种子生活力测定最佳条件:温度为40℃,TTC浓度为0.2%,染色时间为60min。结论:TTC染色率为93%。

Objective：To provide the experiment for consummate the Stellaria dichotoma seed viability certification standard through the target determination of the seed and so on.Methods：To determine the seed viability by the TTC dyeing method.Results：Seed viability determination optimum condition：the temperature is 40 ℃,the TTC density is 0.2%,the dyeing time is 60min.Conclusion：TTC dyeing rate is 93%.