内蒙古包头地区单核细胞趋化因子MCP-1 2518G/A基因多态性与脑梗死的相关性研究

Correlational study between polymorphism of monocyte chemoattractant protein-1(MCP-1) gene-2518G/A and cerebral infarction

目的探讨脑梗死患者单核细胞趋化蛋白-1(MCP-1)基因-2518G/A的基因型及基因频率的分布特点及其与脑梗死(CI)病情轻重的关系。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测148例脑梗死患者和130例正常人的MCP-1的基因型及基因频率分布。回顾病史并结合临床进行统计学分析。结果脑梗死组GG、GA、AA基因型频率分别为35%、46%、19%,G、A等位基因频率分别为58.0%、42.0%;正常对照组GG、GA、AA基因型频率分别为25%、38%、37%,G、A等位基因频率分别为44.0%、56.0%。脑梗死组GG基因型和G等位基因频率显著高于对照组(均P<0.05)。脑梗死组病情程度不同,G、A等位基因频率存在差异。其中轻度组G、A等位基因频率分别47%、53%;重度组G、A等位基因频率分别为72%、28%。脑梗死病情重度组G等位基因频率显著高于轻度组(P<0.05)。结论 MCP-1基因-2518G/A位点的G等位基因可能是脑梗死发病的遗传易感基因,且与脑梗死的病情轻重有关。

Objective To investigate the monocyte chemoattractant protein-1（MCP-1） gene-2518G/A genotype and gene frequency distribution of patients with cerebral infarction and its association with severity.Methods With the technique of polymerase chain reaction restriction fragment length polymorphism（PCR-RFLP）,148 cases of cerebral infarction and 130 normal persons were detected on MCP-1 genotype and gene frequency distribution.Results Genotypes frequency of GG,GA and AA in the group of cerebral infarction was 35%,46% and 19%.G and A allele frequencies were 58% and 42%,respectively.Genotypes frequency of GG,GA,AA in the group of control were 25%,38%,37%,while G,A allele frequencies were 44%,56%.Frequency of GG genotype and G allele in the group of cerebral infarction was significantly higher（P0.05） than control group.Different severity of cerebral infarction group,G,A allele frequency exist differences.In mild group,G,A allele frequencies were 47%,53%;while in severe group G,A allele frequencies were 72%,28%.In severe cerebral infarction group,G allele frequency was significantly higher than the mild group（P0.05）.Conclusion G allele that is located in MCP-1 gene-2518G/A may be a susceptibility gene of cerebral infarction.The MCP-1-2518G allele may be associated with the severity of cerebral infarction.