鬼箭羽饮片中香橙素含量的HPLC测定

Determination of aromadendrin in Euonymus alatus by HPLC

目的:建立鬼箭羽中香橙素含量测定的分析方法。方法:采用Kromasil100-5C18色谱柱(4.6mm×250mm,5μm),以乙腈-0.3%醋酸(24∶76)为流动相,检测波长292nm,流速1.0mL·min-1,检测波长292nm,柱温30℃。结果:香橙素在0.051～2.04μg呈良好的线性关系,r=0.9999。20批鬼箭羽中香橙素质量分数在0.00157%～0.0416%。结论:该方法简单、重复性好,可用于鬼箭羽的质量控制。

Objective：To establish a HPLC method to determine the content of aromadendrin in Euonymus alatus. Method： Kromasil 100-5C18 column was used with a mobile phase composed of acetonitrile-0.3% glacial acetic acid（24∶76）at a flow rate of 1.0 mL·min-1. The detection wavelength was 292 nm and the temperature of column was 30 ℃. Result： The calibration curve was linear in the range of 0.051-2.04 μg for aromadendrin. The correlation coefficient of the calibration curve was 0.999 9. Twenty batches of the crude E. alatus purchased from different areas were determined and the contents of aromadendrin in the twigs of E. alatus were fluctuated from 0.001 57% to 0.041 6%.Conclusion： This method is simple, repeatable and can be used for determination of aromadendrin.