微透析法研究冰片对头孢曲松在大鼠脑纹状体中含量的影响

Microdialysis and HPLC method for determination of concentration of pantoprazole in rat striatum after combined administration with borneol

目的:探索芳香开窍中药冰片对西药头孢曲松在大鼠脑纹状体中浓度的影响及其相关性。方法:采用脑微透析技术采集大鼠脑纹状体内的脑脊液(aCSF)样品,利用高效液相色谱仪结合二极管阵列检测器,检测对照品和样品中头孢曲松的含量,进行统计学分析,比较头孢曲松合用冰片组与单用头孢曲松组大鼠脑纹状体中头孢曲松的浓度。结果:头孢曲松合用冰片组的大鼠脑纹状体内头孢曲松质量浓度13.01～4.43mg·L-1明显高于单用头孢曲松组2.41～0.94mg·L-1。结论:冰片能够促进头孢曲松透过血脑屏障,提高其在纹状体aCSF中的浓度。

Objective：To observe the influence of borneol, a traditional Chinese medicine, on the concentration of ceftriaxone in the rat brain striatum and evaluate the relativity. Method： The sample of cerebrospinal fluid in the rat brain striatum was collected via brain microdialysis technology, and then the contents of ceftriaxone in standard preparation and sample were detected by high efficiency liquid chromatography combined with diode array detector respectively and analyzed statistically. The concentration of ceftriaxone in rat brain striatum in the ceftriaxone ＋ Borneol group was compared with that in the ceftriaxone-only group. Result： The concentration of ceftriaxone in the rat brain in the ceftriaxone ＋ Borneol group（13.01-4.43 mg·L-1） is significantly higher than that in the ceftriaxone-only group（2.41-0.94 mg·L-1）. Conclusion： Borneol can promote ceftriaxone through blood-brain barrier, and increase the concentration thereof in striatum.