荭叶心通软胶囊对小鼠耐缺氧及体外培养心肌细胞的影响

Hongyexintong Soft Capsule on Hypoxia Tolerance in Mice and in Vitro Cultured Myocardial Cells

目的观察荭叶心通软胶囊对小鼠耐缺氧及体外培养心肌细胞的影响。方法夹闭小鼠气管,以不同剂量的荭叶心通软胶囊与普萘诺尔做对照,观察给药后小鼠缺氧心跳停止时间;离体培养大鼠乳鼠原代心肌细胞,以不同剂量的荭叶心通软胶囊与香丹注射液做对照,检测给药后心肌细胞A570和培养上清液中LDH活力。结果荭叶心通软胶囊高、中剂量组能明显延长小鼠气管结扎的心跳时间,增加小鼠心脏耐缺氧能力。荭叶心通软胶囊高剂量组可明显提高心肌细胞A570,降低培养上清液LDH的活力。结论荭叶心通软胶囊具有改善小鼠耐缺氧的作用,对NaCN诱导心肌细胞损伤具有一定的保护作用。

OBJECTIVE To investigate the effect on hypoxia in mice and myocardial cells in vitro changes of Hongyexintong soft capsule.METHODS The hypoxic cardiac arrest time,myocyte primary culture A570 and the LDH activity of mice which were treated by Hongyexintong soft capsule at different doses were observed and compared with that treated by Xiangdan injection.RESULTS The high and middle dose group of Hongyexintong soft capsule could significantly prolong the time mice tracheal ligation of the heartbeat and increase cardiac hypoxia tolerance in mice.The high dose group of Hongyexintong soft capsule could obviously improve the NaCN-induced myocardial cell injury induced by primary A570 due to the decline,reducing the vitality of culture supernatant LDH.CONCLUSION Hongyexintong soft capsule has effect of improving the role of hypoxia in mice and protecting myocardial cell,which injuried by NaCN-induced.