膳食蛋白质对大鼠脂联素及其受体基因表达影响

Effect of dietary protein on expression of adiponectin and its receptors in rats

目的探讨不同膳食蛋白质摄入对大鼠脂联素及其受体表达的影响。方法将36只雄性成年Wistar大鼠按体重随机分为酪蛋白组、大豆蛋白组和谷豆蛋白混合组,喂养12周,观察各组大鼠血脂水平及脂肪组织脂联素、骨骼肌组织脂联素受体1(Adipo R1)和肝脏组织脂联素受体2(Adipo R2)mRNA表达水平。结果大豆蛋白组和谷豆蛋白混合组大鼠脂联素mRNA水平分别为(1.17±0.05)和(1.46±0.02),均高于酪蛋白组(0.79±0.06),AdipoR1 mRNA水平分别为(1.14±0.02)和(1.26±0.05),均高于酪蛋白组(0.89±0.06),谷豆混合蛋白组大鼠脂联素mRNA水平高于大豆蛋白组,差异均有统计学意义(P<0.05);脂联素mRNA水平与血清甘油三脂(TG)水平呈负相关(r=-0.64,P<0.05)。结论膳食蛋白质可影响大鼠脂联素和脂联素受体的表达,大豆蛋白和谷豆混合蛋白相对酪蛋白可上调脂联素及其受体的表达,且谷豆混合蛋白的作用更强。

Objective To explore the effects of different dietary proteins on the expression of adiponectin and its receptors in rats.Methods Thirty-six male adult Wistar rats were randomly divided into casein protein group,soy protein group and the glutelin-soy mixed protein group by body weight and were fed for 12 weeks.Plasma was collected for the determination of blood lipids and adipose tissue,and skeletal muscle and hepatic tissue were detected for the expression of adiponectin,adiponectin receptor 1（AdipoR1） and adiponectin receptor 2（AdipoR2）,respectively.Results Compared to the rats fed with casein protein,the expression of adiponectin in adipose tissue and AdipoR1 in skeletal muscle were significantly higher（P0.05） in other two groups.Furthermore,rats fed with glutein-soy mixed protein had a significantly higher expression of adiponectin in adipose tissue（P0.05） than rats fed with soy protein.In addition,adiponectin expression in adipose tissue was inversely associated with triglyceride level in plasma（r=-0.64,P0.05）.Conclusion Different dietary proteins have distinct effects on the expression of adiponectin and its receptors in rats.Both the soy protein and the glutelin-soy mixed protein can up-regulate the expression of adiponectin and adiponectin receptors,especially the latter.