摘要
目的探讨人乳头瘤病毒(HPV)16/18感染、p53在乳腺浸润性导管癌中的表达及其相互关系。方法采用免疫组化方法和PCR技术,分别检测45例乳腺浸润性导管癌、20例乳腺纤维腺瘤及20例正常乳腺组织中HPV16/18 DNA和p53表达水平。结果乳腺癌组HPV16/18 DNA和p53蛋白的表达率分别为51.1%和46.7%,明显高于乳腺纤维腺瘤组的15.0%和10.0%和正常乳腺组的5.0%和0.0%(P<0.01);有腋淋巴结转移者的表达率明显高于无腋淋巴结转移癌者(P<0.05);随着原发肿瘤局部淋巴结远处转移(TMN)分期的升高,p53蛋白的表达率明显增加(P<0.05);HPV16/18 DNA与p53蛋白的表达有关(2χ=6.517,P<0.05)。结论 HPV感染和p53突变共同参与乳腺癌的发生和发展,HPV感染可能是促进p53突变的重要因素。
Objective To study the infection of human papillomavirus（HPV） 16/18 and its relationship with expression of p53 in invasive ductal carcinoma.Methods The expression of HPV16/18 DNA and p53 in the invasive ductal carcinoma（n=45）,breast fibroadenoma（n=20） and normal breast tissue（n=20） were detected with immunohistochemistry method and polymerase chain reaction.Results The expression rate of HPV 16/18 DNA and p53 protein in invasive ductal carcinoma（51.1% and 46.7%） were significantly higher than those of in fibroadenoma of breast（15.0% and 10.0%） and normal breast tissue（5.0% and 0%,P0.01）,and the expression rate in patients with axillary lymphnode metastasis was significantly higher than those without axillary lymphnode metastasis（P0.05）.The expression rate of p53 protein increased with TMN staging and there was a positive correlation between HPV 16/18 DNA and p53 protein expression（χ2=6.517,P0.05）.Conclusion Both HPV 16/18 infection and p53 mutation are involved in the occurrence and progress of breast carcinoma and HPV 16/18 infection may be the main factor relative to p53 mutation.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2010年第10期1250-1251,共2页
Chinese Journal of Public Health
关键词
乳腺肿瘤
HPV16/18
P53
PCR
免疫组化
breast tumor
HPV16/18
p53
polymerase chain reaction
immunohistochemistry method
