摘要
以野木瓜总皂苷含量为评价指标对比研究了闪式提取、乙醇回流提取和超声波提取3种方法对野木瓜藤茎部位总皂苷的提取效果,并利用正交实验对总皂苷的闪式提取工艺进行了优化.结果表明:闪式提取法所用提取时间短,最佳提取工艺为50倍量50%的乙醇,闪式提取2 min为宜.与乙醇回流提取和超声波提取法相比,闪式提取是一种高效、快速提取野木瓜藤茎部位总皂苷的方法.
Various extraction methods including flash extraction,ethanol refluxing extraction and ultrasonic-assisted extraction were compared for the extraction of total saponins from the rattan of Stauntonia chinensis DC.with the content of the total saponins of Stauntonia chinensis DC.as index.The conditions of flash exaction were optimized by orthogonal test.The results show that the flash extraction requires less time and the optimum extraction conditions were as follows： the plant material was extracted by flash extractor with 50 folds amount of 50 % alcohol for 2 min.The flash extraction technology is an efficient and rapid method for the extraction of the total saponins from the rattan of Stauntonia chinensis DC.,compared with ethanol refluxing extraction and ultrasonic-assisted extraction.
出处
《中南民族大学学报（自然科学版）》
CAS
2010年第3期29-32,共4页
Journal of South-Central University for Nationalities：Natural Science Edition
基金
中南民族大学植物遗传与发育创新团队资助项目
关键词
野木瓜
皂苷
闪式提取
Stauntonia chinensis DC.
saponins
flash extraction
