自制枝莲清肝胶囊抑制乙型肝炎病毒活性的体外实验

The inhibitory effect of Zhilianqinggan on hepatitis B virus in vitro

目的观察枝莲清肝胶囊抑制乙型肝炎病毒(HBV)的活性。方法以HepG2.117细胞为模型,分为3组:空白对照组、枝莲清肝组(1000μg/ml)、拉米夫定组(5μg/ml),用酶联免疫试验法(ELISA)检测细胞培养上清液中HBsAg、HBeAg的变化,用荧光定量PCR法检测细胞培养上清液中HBV DNA的变化,以MTT比色法观察药物对细胞的毒性作用。结果细胞毒性实验显示,2个药物组与空白对照组比较,OD值差异无统计学意义(P>0.05),表明2种药物对HepG2.117细胞的生长无毒性作用。枝莲清肝组24h和72 h对HBsAg的抑制率分别达30.1%和36.3%,对HBeAg的抑制率分别达6.4%和26.7%;PCR检测显示枝莲清肝胶囊能降低HBV DNA水平。除72 h时对HBsAg的抑制率低于拉米夫定组外(P<0.05),2组其余指标比较差异无统计学意义(P>0.05)。结论枝莲清肝胶囊在1mg/ml浓度下有抑制HBV DNA和HBsAg、HBeAg的作用。

Objective To observe the inhibitory effect of Zhilianqinggan（ZLQG） on hepatitis B virus. Methods HepG2.117 cells were divided into 3 groups：blank group,Zkilianqinggan group（1000μg/ml） and lamivudine group（5μg/ml）.Use ELISA to detect the change of HBsAg,HBeAg in cell supernatant.Use PCR to detect the change of HBV DNA.Use MTT to observe the toxicity of the drug.Results It had no statistics difference of OD between experiment group and blank group in the drug toxicity experiment（P0.05）,and it indicated the drug had no toxicity to the growth of the Hep G2.117 cell,and the inhibitory rates of HBsAg separately were 30.1% and 36.3%by ZLQG at the concentration of 1 mg/ml in 24h and 72h,the inhibitory rates of HBeAg separately were 6.4%and 26.7%,PCR detection showed ZLQG could decrease the level of HBV DNA,no difference was found between two groups（P0.05）,except for inhibition of HBsAg at 72h.Conclusion The ZLQG has the inhibit contribution to HBV DNA and HBsAg,HBeAg.