多药耐药鲍氏不动杆菌耐药性与转座子及插入序列遗传标记研究

Drug Resistance and Transposon and Insertion Sequence of Multi-drug Resistant Acinetobacter baumannii

目的探讨多耐药鲍氏不动杆菌耐药性与转座子、插入序列等遗传原件之间的关系。方法 K-B法进行药物敏感试验,PCR进行转座子、插入序列遗传标记检测,并测序。结果临床分离的鲍氏不动杆菌对12种常见抗菌药物耐药率分别为头孢噻肟78.9%、头孢他啶63.1%、头孢吡肟78.9%、头孢西丁100.0%、亚胺培南73.7%、美罗培南73.7%、氨苄西林/舒巴坦78.9%、阿莫西林/克拉维酸78.9%、阿米卡星52.6%、庆大霉素57.8%、环丙沙星78.9%、四环素78.9%;转座子tnp513和插入序列遗传标记ISaba1、IS26检出率分别为47.4%、78.9%、47.4%。结论多药耐药鲍氏不动杆菌的耐药性与转座子和插入序列遗传标记有一定的关系。

OBJECTIVE To explore the relationship between resistance of multidrug-resistant Acinetobacter baumannii and transposon and insertion sequence.METHODS K-B method was used to detect the resistance.PCR was used to analyze the transposon and insertion sequence.RESULTS The resistant rates of A.baumannii were cefotaxime 78.9%,ceftazidime 63.1%,cefepime 78.9%,ceftazidime 100.0%,imipenem 73.7%,meropenem 73.7%,ampicillin 78.9%,amoxicillin 78.9%,amikacin 52.6%,gentamicin 57.8%,ciprofloxacin 78.9% and tetraycline 78.9%,respectively.The detection rate of tnp513 was 47.4%,Isaba1 was 78.9% and IS 26 was 47.4%.CONCLUSION The resistance of multi-drug resistant A.baumannii is associated with transposon and insertion sequence.