湿地松松针中鞣质的提取与分离工艺研究

Study on Extraction and Isolation of Tannin from Pine Needles of Pinus elliottii Engelm.

通过单因素试验法对湿地松松针提取物得率及提取物含鞣质量的影响因素进行了研究,获得了湿地松松针中鞣质提取的最佳条件:提取剂为水,提取温度120℃,提取时间5 h,液料比14∶1(mL∶g),pH值为10。在此条件下,湿地松松针总提取物得率为17.69%,提取物中含鞣质29.17%。同时,利用高效液相色谱(HPLC)对没食子酸进行了初步分离,确定了没食子酸的分离条件,并测定了鞣质加酸分解后没食子酸含量变化,证明提取物中可能含有可分解的水解鞣质或复合鞣质。

Process of extracting tannin from pine needles of Pinus elliottii Engelm.was studied.Five main factors were studied on the yield of pine needle extract and tannin.By single factor experiments,the optimal parameters were confirmed as follows： extracting time 5h at 120℃ with ratio of liquid to solid 14∶1（mL∶g）,and pH value 10.The yield of extract was 17.69 % and tannin was 29.17 % in the extract.Gallic acid and other compositions can be well separated by HPLC technique.The experiment proved that extracts may contain hydrolysable tannin or complex tannins.