摘要
以对醛基苯甲酸和N,N-二甲基苯胺为原料,合成了羧化无色孔雀石绿和羧化孔雀石绿;通过薄层色谱分离纯化了合成产物,对各组分进行了高效液相色谱-质谱鉴定,结果表明m/z为375.2和373.2的碎片峰分别为羧化无色孔雀石绿的[M+H]+和羧化孔雀石绿的[M]+准离子峰;将合成的羧化半抗原通过碳化二亚胺与阳离子化BSA偶联,制备完全抗原,经紫外-可见光谱分析计算,两种完全抗原中的羧化无色孔雀石绿和羧化孔雀石绿与BSA的摩尔比分别为9.2∶1和11.1∶1。完全抗原的成功构建为后续抗体制备奠定了物质基础。
In this paper, 4-formylbenzoic acid and N, N-dimethylaniline were used to synthesize carboxyleucomalachite green （CLMG） and carboxymalachite green （CMG）. The products were separated and purified by thin layer chromatography, and identified by high performance liquid chromatography-mass spectrum. The results showed that m / z 375.2 and m / z 373.2 were attributed to CLMG （M＋H）＋ and CMG （M）＋ respectively. The complete antigens were prepared by conjugating carboxyhaptens on cationized BSA （cBSA） with EDC and analyzed by ultraviolet-visible spectrum. The molar ratios of two kinds of complete antigens （CLMG-cBSA and CMG-cBSA） were 9.2∶1 and 11.1∶1 respectively. The successful preparation of complete antigens was the foundation for further producing antibodies against leucomalachite green and malachite green.
出处
《湖北农业科学》
北大核心
2010年第10期2541-2544,2570,共5页
Hubei Agricultural Sciences
基金
湖北省自然科学基金项目(2008CD2010)
湖北省农业科技创新中心资助项目(2007-620-001-03)
