枯草芽孢杆菌对草鱼生长性能与肠道黏膜形态的影响

Effects of Bacillus subtilis on growth performance and microvill of grass carp

为了研究枯草芽孢杆菌对草鱼生长性能的影响,选取初均重(69.51±6.29)g的草鱼240尾,随机分为5组,每组3个重复,每个重复16尾鱼,分别饲喂基础饲料添加0、1000、1500、2000mg/kg和2500mg/kg枯草芽孢杆菌的试验饲料,经过70d的池塘网箱养殖试验,结果显示:(1)添加枯草芽孢杆菌能够提高草鱼的特定生长率(SGR),降低饲料系数(FCR),其中2000mg/kg组与对照组差异显著(P<0.05);(2)各试验组肠道皱襞高度均高于对照组,其中1500mg/kg和2500mg/kg组显著高于对照组(P<0.05);肠道微绒毛高度基本高于对照组,其中1000mg/kg和2500mg/kg组显著高于对照组(P<0.05),微绒毛宽度均显著高于对照组(P<0.05),微绒毛密度均高于对照组(P>0.05)。以上结果表明:枯草芽孢杆菌能够通过改善草鱼肠道黏膜形态,促进草鱼对饲料的吸收利用,从而促进草鱼生长。

The aim of this experiment was to investigate the effects of Bacillus subtilis on growth performance of grass carp.240 grass carp with an average body weight of （69.51±6.29）g were randomly allocated into 5 groups with 3 replicates of 16 fishes.The control group was fed with the basal diet,while trial groups were fed with the basal diet added with 1000,1500,2000 mg/kg and 2500 mg/kg Bacillus subtilis respectively.The experiment lasted for ？0 days.The results showed that： （1）compared with the control group, specific growth rate （SGR）and feed conversion ratio （FCR）were improved by adding different quantities of Bacillus subtilis ,and the group of 2000 mg/kg was increased significantly （P 〈 0.05）.（2） The intestinal foldings were higher than that of the control group, and group 1500 mg/kg and 2500 mg/kg were significantly higher.And the height of intestinal microvii1 of the group of 1000 mg/kg and 2500 mg/kg were improved significantly （P 〈 0.05）.The width of microvill were improved significantly（P 〈 0.05 ）, and the density of microvill were improved（P 〉 0.05）. In conclusion,Bacillus subtilis could improve the growth performance by improving the intestinal mucosal morphology and promoting absorption and utilization of grass carp.