米诺环素对缺血再灌注大鼠脑组织CD11b和半胱氨酸天冬氨酸蛋白酶3表达的影响

Influence of minocycline on expression of CD11b and caspase-3 in rat brain after focal cerebral ischemia-reperfusion

目的观察米诺环素对缺血再灌注大鼠脑组织CD11b、半胱氨酸天冬氨酸蛋白酶3(caspase-3)表达的影响。方法 30只雄性SD大鼠随机分为:假手术组、生理盐水组、预处理组、缺血30 min组、缺血4 h组。每组6只。采用HE染色观察大鼠脑组织的病理改变,免疫组织化学法检测大鼠脑组织CD11b、caspase-3的表达,用图像分析仪测定吸光度值。结果假手术组可见少量CD11b和caspase-3表达,生理盐水组CD11b和caspase-3表达增多。生理盐水组较预处理组、缺血30 min组及缺血4 h组CD11b和caspase-3的吸光度值明显升高,阳性细胞数明显增多,差异有统计学意义(P<0.05,P<0.01)。结论米诺环素能在一定程度上抑制脑缺血后CD11b及caspase-3的表达,发挥脑保护作用。

Objective To observe the influence of minocycline on the expressin of CD11b and caspase-3 in the rat brain after focal cerebral ischemia-reperfusion. Methods Thirty healthy male Sprague-Dawley rats were randomly divided into sham-operation group, control group, minocycline pretreatment group,ischemia 30 rain-group in which the rats were administered with minocy- cline 30 minute after ischemia, ischemia 4 hour group in which the rats were administered with minoeycline 4 hours after ischemia, each group contained 6 rats. The expression of CDllh and caspase-3 was measured by immunohistochemistry. The pathological change was observed using hematoxylin-eosin staining. The optic absorbance value was measured by image analyzer. Results The expression of CD11h and caspase-3 was very little in sham-operation group. The expression of CDllb and caspase-3 obviously increased in control group. The optic absorhance values of CDllb and caspase-3 significantly decreased and the number of positive CD11h and caspase-3 cells significantly reduced in minoeycline pretreatment group, 30 rain-group and 4 h-group, compared with control group （P 〈0.05, P 〈 0.01）. Conclusion Minocycline could decrease the expression of CDllb and caspase-3 to certain extent in rats after focal cerebral ischemia-reperfusion and could protect brain function.