显微图像和SEM图像在褶蛛外生殖器形态学研究中的比较

Comparisons of Microscopy and SEM Images in Morphological Study of Micronetine Genitalia

显微观察一直是基于形态特征进行蜘蛛分类学研究的主要手段,可以为蜘蛛分类提供所需要的鉴定特征.而深入研究蜘蛛复杂的外生殖器形态特征时,由于显微图像在高倍放大条件下,景深变小,导致三维立体信息丢失.相比之下,SEM图像在表现微小、复杂结构的细节和三维形态上,更胜一筹.然而,扫描电镜无法显示内部结构的局限使其在进行深入的比较形态学研究中,并不能完全取代显微观察.本文结合Crispiphantes rhomboidea外生殖器的形态学研究,针对显微图像和SEM图像在研究微小、复杂的褶蛛外生殖器结构中所发挥的作用进行了比较,分析了二者的优势和局限.结果显示综合显微图像和SEM图像信息可以最大限度的挖掘蜘蛛复杂的外生殖器形态结构中所蕴涵的系统发育信息,有助于我们进行同源结构的比较.

Microscopy has been widely used in spider taxonomy,providing morphological diagnosis for classification.When study spider genitalic morphology in detail,the microscope images under high magnification would become less focus depth that lead to loss of the three dimensional information.SEM images are better in revealing the small and complex structures in detail,but can not take microscopy instead as they are limited in showing the internal structures.In the present paper,based on the study of epigynal morphology of Crispiphantes rhomboidea,we compared the functions of microscopy and SEM images in morphological study of small and complex micronetine genitalia,discussed the advantages and limitations of both.The results show that combination of the information from both microscopy and SEM images will help to find maximum phylogenetic information included in the spider complex genitalic morphology.