摘要
目的研究过氧化体增殖剂激活受体γ（PPARγ）基因Pro12Ala多态性与冠心病之间的相关性。方法180例入选行冠状动脉造影患者均于入院后12h内空腹晨起静脉采血，从全血中抽提基因组DNA，多聚酶链反应．限制性片段长度多态性（PCR-RFLP）技术分析PPA聊基因Prol2Ala多态性在上海汉族冠心病人群中的发生频率和基因型分布。根据冠状动脉造影结果采用Gensini积分方法对冠状动脉进行评分。结果检出冠心病135例（冠心病组），其中冠状动脉单支病变52例，双支病变40例，多支病变43例；冠状动脉造影正常45例（对照组）。①冠心病组与对照组P12／P12基因型分别占92．6％（125／135）与93．3％（42／45），A12／P12基因型占7．4％（10／135）与6．7％（3／45），2组差异无统计学意义（P=0．548）。亚组分析中，对照组：P12／P12与A12／P12基因型为93．3％（42／45）与6．7％（3／45），单支病变组：P12／P12与A12／P12基因型90．4％（42／52）与9．6％（5／52），双支病变组：P12／P12与A12／P12基因型为90．0％（36／40）与10．0％（4／40），多支病变组P12／P12与A12／P12基因型为97．7％（42／43）与2．3％（1／43）。各组之间无统计学意义（P〉0．05）。②按照冠状动脉Gensini积分方法，不同的冠状动脉管腔狭窄组之间，P12／P12和A12／P12基因型积分差异无统计学意义（P=0．246）。③肥胖的冠心病患者中A12／P12基因型明显高于非肥胖患者（P=0．028）。④各基因型临床特征的比较发现，PPARγ基因B外显子BstuI酶切位点P12A基因型体重指数、腰臀比和总胆固醇显著高于P12P基因型（P均〈0．05）。结论PPA脚基因Pro12A1a多态性可能与中国汉族人群冠心病发生无关。肥胖的冠心病患者中A12／P12基因型明显高于非肥胖者。PPARv基因Prol2Ala多态性可能与肥胖及脂质代谢有关。
Objective To investigate the association of PPAR gamma Prol2Ala polymorphisms with conroy heart disease（CHD）. Methods One hundred eighty impatients were recruited in the study. Venous blood samples were collected after 12 h of fasting from all patients. DNA was extracted and Pro2Ala polymorphism in the PPAR gamma 2 genes was genotyped through the PCR-RFLP. The frequency and distribution of Pro2Ala polymorphism in Chinese Han population with CHD were analyzed. Gensini Score based on coronarography were used to grade the coronary. Results There were a total of 135 CHD patients, of which 52 were affected with single coronary lesion ,43 with multi-coronary lesion,and 45 were normal. The frequency of P12/P12, A12/P12 in the coronary lesion group were 92.6% （125/135） and 7.4% （10/135） which were similar to that of 93.3% （42/45） and 6.7% （3/45） （P =0. 548）. In the single coronary lesion group,the frequencies of P12/P12 and A12/P12 were 94.0% （42/52） and 9.6% （5/52） ,and 90.0% （36/40） and 10.0% （4/40） in the bi-coronary lesion group,97.7% （42/43） and 2.3% （ 1/43 ） in the multi-coronary lesion group, with no significant difference among the three groups （P 〉 0.05 ）. There were no significant difference on the frequency of P12/P12 and A12/P12 between various subgroups defined according to Gensini score （P=0. 246）. Compared to the frequency in non-obese, the frequency of A12/P12 were significantly higher in the obese （ P = 0. 028 ）. In PPAR gamma 2 genes B the Exton BstuI enzyme restriction site genes,BMI,waist-hip ratio and total cholesterol in the carriers of P12A genotype were significantly higher than those in the P12P genotype carriers （ P 〈 0.05 ）. Conclusions PPAR gamma Prol2Ala polymorphisms might not be associated with CHD in Chinese Han population. The frequency of A12/P12 in obese is significantly higher than that in non-obese. PPAR gamma Prol2Ala polymorphisms might be associated with adiposity and lipid metabolism.
出处
《中国综合临床》
2010年第10期1043-1046,共4页
Clinical Medicine of China
